FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_A6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_A6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11229646
Sequences flagged as poor quality0
Sequence length110
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGA343110.30553946224128525No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC315580.2810239966602687No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC293120.26102336618625377No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC290870.25901974113876786No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTT289460.25776413610901006No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC269210.23973151068163678No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGC240530.2141919700763497No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACTCCTACCTATC231080.20577674487690886No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG215280.19170684454345222No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGT215200.19163560454176384No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT207320.1846184643754576No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTT201950.17983647926212457No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA199360.177530084207463No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT198390.17666629918699128No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGT196880.17532164415512297No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGG195000.17364750411544586No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCC190290.169453249016042No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCC186500.16607825393605463No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGG182430.1624539188501579No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA180710.16092225881385755No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC178460.15891863376637164No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC177210.15780550873999055No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAATTTTTCGTTC176070.15679033871593104No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG174580.1554634936844848No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG174580.1554634936844848No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACG174520.15541006368321852No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA171050.1523200286099847No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACG169720.15113566358191521No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTC168160.14974648354899167No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTCATGCGCCGAA163660.14573923345401985No Hit
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTC162110.14435895842130733No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGA159210.14177650836010325No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC159070.14165183835714856No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTCATGTTAGCTT158520.1411620633455409No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCT153800.13695890324592602No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAG152510.13581015821870077No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCT152510.13581015821870077No Hit
CATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT151750.13513337820266108No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT150520.1340380631767021No Hit
GATGCAGAGTGGGGTTTTGCAGTCCTTAGCTGTTACAGAAATTAAGTATT150170.1337263881693154No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA148910.13260435814272328No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGG147510.1313576581131765No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACT145980.12999519308088606No Hit
CCTATTTATGGGGGTTTAGTATTGATTGTTAGCGGTGTGGTCGGGTGTGT145190.12929169806421326No Hit
GGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTTG144130.1283477680418421No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG143670.12793813803213386No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGATAAGGGTGGA142150.12658457800005452No Hit
TTTGTTTATGGGGTGATGTGAGCCCGTCTAAACATTTTCAGTGTATTGCT141060.12561393297705023No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAGTCCC140670.12526663796881932No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAGGCTTACTAGA138890.12368154793125267No Hit
CCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAA137630.12255951790466058No Hit
CCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGCTGACGCCATA136910.12191835788946508No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAG135680.1208230428635061No Hit
GCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGAT135560.12071618286097353No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTCTGTATGTCTC134960.12018188284831062No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTAC134440.1197188228373361No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCTTGGAAAAAGG134390.11967429783628085No Hit
AGGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTT134200.11950510283227093No Hit
CCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTCAATCACAC132820.11827621280314624No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC130690.11637944775819292No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGGTTTCT130610.11630820775650452No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCG129560.11537318273434442No Hit
CCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACCTACGCCTA128340.11428677270859651No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGACTTTTTCAAAA127120.1132003626828486No Hit
GCCATCATGACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGA126810.1129243076763061No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCA126540.1126838726706078No Hit
TCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACC126490.11263934766955254No Hit
AGTAAGGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTC126470.11262153766913045No Hit
AGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAA124810.1111433076340964No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTTCACTGATCTCGTA124350.11073367762438817No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTAT122540.10912187258618837No Hit
CTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTCAGGCGTTTGT121810.10847180757078184No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG119510.10642365752224069No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGA118780.10577359250683413No Hit
ATTGGTGCGGGGGCTTTGTATGATTATGGGCGTTGATTAGTAGTAGTTAC117540.10466937248066413No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC117480.10461594247939783No Hit
GGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAG117240.10440222247433267No Hit
CTTATTTCTAGGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATT116860.10406383246631284No Hit
GGTGATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAGAATAGGAGGT115500.10285275243761025No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTA114530.10198896741713853No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTC114350.10182867741333965No Hit
CCTAGATAGGGGATTGTGCGGTGTGTGATGCTAGGGTAGAATCCGAGTAT112740.10039497237936085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)