FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_A5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_A5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11821021
Sequences flagged as poor quality0
Sequence length110
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC310210.2624223406759873No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC292500.2474405552616817No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTT269260.22778066293935184No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC242890.20547294518806794No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC231570.1958967842117868No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA227080.19209846594469293No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGC214430.18139719064876036No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGG212200.17951072077445762No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAATTTTTCGTTC195430.16532412893945456No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT193550.16373374178084954No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTCATGCGCCGAA188590.15953782672410446No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA186890.15809971067642972No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGA180740.15289711438631232No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG178900.15134056525235848No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGT176090.1489634440206138No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTT173760.14699237908468313No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG172600.14601107636979918No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA170390.14414152550782205No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC168290.1423650292136356No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCC164500.13915887637793725No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC161520.13663794354142506No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAG156330.13224745984293573No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGACTTTTTCAAAA155980.131951377127238No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGATAAGGGTGGA152580.12907514503188855No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGGTTTCT152030.12860987219293496No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCC147750.12498920355525973No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAGGCTTACTAGA144000.12181688874421254No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCTTGGAAAAAGG143300.12122472331281704No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAGTCCC142920.12090326207863093No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT142230.12031955615339825No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGA139640.11812854405723498No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC139530.11803548948944426No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTC139010.11759559516897905No Hit
CATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT133840.11322203048281532No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG132140.11178391443514059No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCT129090.10920376505548886No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAG128680.10885692530281438No Hit
CTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTCAGGCGTTTGT126920.1073680522181629No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACG125300.10599761221979048No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTC124340.10518549962816241No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTCATGTTAGCTT121040.10239386259444087No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGT120460.1019032112369989No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC120080.10158175000281279No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACT119680.10124336975630109No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT118720.100431257164673No Hit
ATTGGTGCGGGGGCTTTGTATGATTATGGGCGTTGATTAGTAGTAGTTAC118320.10009287691816131No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)