FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_A4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_A4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6742708
Sequences flagged as poor quality0
Sequence length110
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC242110.35906938280584005No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC227400.3372532222958491No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTT223720.3317954744592232No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC211130.31312345128989716No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC189850.2815634311911475No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGG170760.2532513642886508No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGC169260.25102673881176524No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGA169190.25092292295617724No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA166600.24708173629942157No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCC150540.2232634128602336No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT149270.22137989662313717No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAATTTTTCGTTC148660.22047521559587038No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA147650.2189773011081008No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGT146770.2176721874949946No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTT144400.21415727924151542No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG143400.21267419559025838No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTCATGCGCCGAA140550.20844740718417584No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG138700.20570370242935032No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC133220.19757640402046178No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA130330.19329029226832897No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGT127010.18836645454615564No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC123630.18335363180490685No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAG121890.18077306625171963No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT120640.17891921168764835No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCC119520.17725815799824046No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGGTTTCT119410.1770950187966022No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAGGCTTACTAGA117440.17417334400362583No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTC116710.1730906929382082No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGACTTTTTCAAAA116440.1726902603523688No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCT116070.1721415194014037No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACG114030.16911602875283935No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG112700.1671435274966675No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGATAAGGGTGGA110390.16371760426226376No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACG108810.16137433209327767No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGA107110.15885308988614072No Hit
GATGCAGAGTGGGGTTTTGCAGTCCTTAGCTGTTACAGAAATTAAGTATT106790.15837850311773846No Hit
CATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT106150.15742932958093395No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACTCCTACCTATC105910.15707338950463226No Hit
CTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTCAGGCGTTTGT105830.1569547428125317No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT105380.15628735516946604No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTCATGTTAGCTT104580.1551008882484604No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTC102080.15139317912031783No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC102020.1513041941012424No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAG101190.15007323467069905No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACT100950.1497172945943974No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA100910.1496579712483471No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAGTCCC100570.14915372280691971No Hit
ATTGGTGCGGGGGCTTTGTATGATTATGGGCGTTGATTAGTAGTAGTTAC98730.14642484888860677No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCG97430.14449684014197262No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCTTGGAAAAAGG97420.14448200930546007No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTAC95080.1410115935615186No Hit
AGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAA95040.14095227021546833No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGA94790.14058149930265407No Hit
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTC93240.13828271964320568No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGA92870.13773397869224058No Hit
CCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGCTGACGCCATA91980.1364140342426218No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCA91430.13559833823443046No Hit
GGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTTG91350.13547969154232986No Hit
TTTGTTTATGGGGTGATGTGAGCCCGTCTAAACATTTTCAGTGTATTGCT91060.13504959728346533No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGG90780.13463433386111337No Hit
CTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTTGCGCTGCATGT90760.13460467218808825No Hit
CCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACCTACGCCTA89850.13325506606544432No Hit
CCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATC89170.13224656918258956No Hit
CCTATTTATGGGGGTTTAGTATTGATTGTTAGCGGTGTGGTCGGGTGTGT89000.13199444496187585No Hit
CCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAA88530.13129739564578505No Hit
GCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGAT86470.12824224332419557No Hit
GCAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGT86410.12815325830512014No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCT85990.12753036317159216No Hit
AGTAAGGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTC85690.12708543807621508No Hit
AGGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTT85230.12640321959663683No Hit
CCTAGATAGGGGATTGTGCGGTGTGTGATGCTAGGGTAGAATCCGAGTAT85200.12635872708709914No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTT85100.12621041872197342No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG85060.12615109537592314No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC83980.12454936503256556No Hit
CCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCGTACTATA83320.12357052982273592No Hit
GCCTAACCCCTGTCTTTAGATTTACAGTCCAATGCTTCACTCAGCCATTT82780.12276966465105711No Hit
AGGGAGAGCTGGGTTGTTTGGGTTGTGGCTCAGTGTCAGTTCGAGATAAT82740.12271034130500683No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGG81540.12093064092349838No Hit
GGTAAGGATGGGGGGAATTAGGGAAGTCAGGGTTAGGGTGGTTATAGTAG80760.1197738356755179No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC80700.11968485065644249No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC80690.11967001981992992No Hit
GGTGATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAGAATAGGAGGT80190.1189284779943014No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT79440.11781616525585863No Hit
CCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTTAACAGTACAT78850.11694114590161697No Hit
AGCATTAGCAGGAATACCTTTCCTCACAGGTTTCTACTCCAAAGACCACA78700.11671868335392842No Hit
TCTATTGATGAGGGTCTTACTCTTTTAGTATAAATAGTACCGTTAACTTC78440.11633308160460158No Hit
GCCATCATGACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGA77890.11551738559641023No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTAT77810.11539873890430966No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTC77640.11514661468359597No Hit
CCTTAACCTCTACTTCTACCTACGCCTAATCTACTCCACCTCAATCACAC76970.11415294863725375No Hit
AGCTATTAGAAGGATTATGGATGCGGTTGCTTGCGTGAGGAAATACTTGA76900.11404913278166577No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTCTGTATGTCTC76450.1133817451386001No Hit
GTAAGGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACT75650.11219527821759448No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG75600.11212112403503162No Hit
GCCAATGGTGAGGGAGGTTGAAGTGAGAGGTATGGTTTTGAGTAGTCCTC74870.111038472969614No Hit
GGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAG74600.11063804038377459No Hit
CGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAG73910.10961471266440724No Hit
GGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAG73230.10860621578155245No Hit
TCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACC72350.10730110216844627No Hit
ACCCCTTCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATC72080.10690066958260686No Hit
GGTGAGGGGTGGCTTTGGAGTTGCAGTTGATGTGTGATAGTTGAGGGTTG71890.10661888368886803No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTA71790.10647057532374234No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG71680.10630743612210405No Hit
GCTTACGACCCCTTATTTACCGAGAAAGCTCACAAGAACTGCTAACTCAT70560.10464638243269618No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCAT69850.10359339304030368No Hit
ACTATTTATGTGCTCCGGGTCCATCATCCACAACCTTAACAATGAACAAG69830.10356373136727855No Hit
AGAAATAAGGGGGTTTAAGCTCCTATTATTTACTCTATCAAAGTAACTCT69310.10279252786862489No Hit
GATGTGGGGAGGGGTGTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGT69050.10240692611929807No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGC68810.10205098604299638No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT68770.1019916626969461No Hit
CCGCACCAATAGGATCCTCCCGAATCAACCCTGACCCCTCTCCTTCATAA68350.10136876756341813No Hit
GGTCGAAGGGGGTTCGGTTGGTCTCTGCTAGTGTGGAGATAAATCATATT68240.10120562836177986No Hit
CTTATTTCTAGGACTATGAGAATCGAACCCATCCCTGAGAATCCAAAATT68200.10114630501572959No Hit
CTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAG67830.10059756406476449No Hit
CCTCAACTGCCTGCTATGATGGATAAGATTGAGAGAGTGAGGAGAAGGCT67480.10007848478682452No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)