FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15926490
Sequences flagged as poor quality0
Sequence length110
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC547280.3436287593813829No Hit
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC546590.34319551891220224No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTT472070.29640554824069837No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC434890.27306079368398184No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC433830.2723952358617624No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGC386240.2425142011830604No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGA380380.2388347966187151No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT378880.2378929695118008No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA342170.21484332078191742No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGG341290.2142907822125277No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA334110.2097825697940978No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC329940.20716429043687595No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCC326400.2049415784645581No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG322710.20262468378154885No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAATTTTTCGTTC322420.20244259720754543No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTCATGCGCCGAA306380.1923713260109415No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC287200.18032849673719697No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG283290.17787346741184026No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT281130.17651723637788364No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGT277820.1744389378952927No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTT276840.17382361085210865No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAG271690.17059000445170278No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA262380.1647443975414545No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGACTTTTTCAAAA252270.15839648284085192No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCT244870.15375013578007457No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGGTTTCT240720.1511444141176116No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCC240030.150711173648431No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACTCCTACCTATC239590.15043490436373613No Hit
CATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT237950.1494051733935098No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGT236250.14833776933900691No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTC235340.14776639422747886No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACG235190.14767221151678744No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG228270.14332724913022268No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAGGCTTACTAGA228070.14320167218263408No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAG224210.14077803709417455No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTC224090.1407026909256214No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGA221760.13923971948621447No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGATAAGGGTGGA219050.13753815184638926No Hit
GATGCAGAGTGGGGTTTTGCAGTCCTTAGCTGTTACAGAAATTAAGTATT218990.13750047876211266No Hit
CCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGCTGACGCCATA218980.13749419991473324No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA214750.13483824747323483No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT210080.13190602574704155No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC207000.12997214075417748No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAGTCCC205280.1288921790049157No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACG204490.12839615006194083No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC200310.12577159185733958No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGA200230.12572136107830414No Hit
TTTGTTTATGGGGTGATGTGAGCCCGTCTAAACATTTTCAGTGTATTGCT198000.1243211781126915No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCA195680.122864485520664No Hit
CTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTCAGGCGTTTGT195080.12248775467789827No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGG194840.12233706234079197No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCG193490.12148941794456908No Hit
CCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATC191830.1204471292795839No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCTTGGAAAAAGG190490.11960576373074043No Hit
CTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTTGCGCTGCATGT189330.11887741743472667No Hit
CCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTTAACAGTACAT189200.1187957924187941No Hit
GCCTAACCCCTGTCTTTAGATTTACAGTCCAATGCTTCACTCAGCCATTT188200.11816790768085121No Hit
ATTGGTGCGGGGGCTTTGTATGATTATGGGCGTTGATTAGTAGTAGTTAC187480.11771583066953234No Hit
GCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGAT186740.11725119596345458No Hit
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTC186320.1169874843735186No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTAC184980.11614611882467513No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG184900.1160958880456397No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACT183850.11543660907079967No Hit
GGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTTG182450.11455757043767961No Hit
TCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACC180580.11338342597772641No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC180570.11337714713034698No Hit
CCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACCTACGCCTA179840.11291879127164868No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTCATGTTAGCTT178930.11234741616012066No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGC178200.11188906030142234No Hit
GCAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGT176610.11089072356809315No Hit
AGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAA176410.11076514662050457No Hit
CCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAA176180.11062073313077771No Hit
AGTAAGGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTC175860.110419810014636No Hit
CCTATTTATGGGGGTTTAGTATTGATTGTTAGCGGTGTGGTCGGGTGTGT175830.11040097347249771No Hit
CCTCAGATAGGGGTCCCTTGATCACCATCCTCCGTGAAATCAATATCCCG172900.10856127119032505No Hit
GGTAAGGATGGGGGGAATTAGGGAAGTCAGGGTTAGGGTGGTTATAGTAG171720.10782036719955243No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTT171600.1077450210309993No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCT170960.10734317479871584No Hit
GGTGATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAGAATAGGAGGT169520.10643902077607809No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTAT168110.10555370329557862No Hit
CGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAG167770.10534022248467804No Hit
CCTAGATAGGGGATTGTGCGGTGTGTGATGCTAGGGTAGAATCCGAGTAT166330.10443606846204029No Hit
GCCATCATGACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGA165680.1040279433823774No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG164260.1031363470544985No Hit
AGGGAGAGCTGGGTTGTTTGGGTTGTGGCTCAGTGTCAGTTCGAGATAAT162570.10207522184737504No Hit
AGCATTAGCAGGAATACCTTTCCTCACAGGTTTCTACTCCAAAGACCACA161630.10148501019370872No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGG160300.10064992349224468No Hit
AGCTATTAGAAGGATTATGGATGCGGTTGCTTGCGTGAGGAAATACTTGA160120.10053690423941497No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC159550.1001790099387875No Hit
GGTGAGGGGTGGCTTTGGAGTTGCAGTTGATGTGTGATAGTTGAGGGTTG159290.10001575990692237No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)