FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_A2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_A2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268943
Sequences flagged as poor quality0
Sequence length110
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAGAACAGGGTTTGTTAAGATGGCAGAGCCCGGTAATCGCATAAAAC87510.3856861983751905No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC81820.36060844190444624No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTT75390.3322692548909338No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTAC66890.2948068770348131No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC58710.2587548475215111No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGG58540.2580055999643887No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA57440.25315752753594956No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT54790.24147808032198254No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGC52820.23279562333650516No Hit
ACAACGTTGGGGCCTTTGCGTAGTTGTATATAGCCTAGAATTTTTCGTTC51340.22627276225096882No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG49690.21900065360831011No Hit
CGAATAAGGAGGCTTAGAGCTGTGCCTAGGACTCCAGCTCATGCGCCGAA49460.2179869657369092No Hit
ACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCC48700.2146373884227149No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGA46940.20688047253721226No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGT46070.203046087980174No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA45620.20106278562308527No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGACTTTTTCAAAA45100.19877096956600498No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTT44670.19687581398034237No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA44650.1967876672089162No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG44300.19524509870895831No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC43310.19088183352336308No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC42030.18524044015208846No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAG40990.1806568080379278No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT40190.17713093718088113No Hit
TGTGATTGGTGGGTCATTATGTGTTGTCGTGCAGGTAGAGGCTTACTAGA40030.17642576300947183No Hit
CACCATTGGCAGCCTAGCATTAGCAGGAATACCTTTCCTCACAGGTTTCT39890.17580873560948865No Hit
ACTCTGCATCAACTGAACGCAAATCAGCCACTTTAATTAAGCTAAGCCCT39380.1735609929381214No Hit
ACTTCTAACCTCCCTGTTCTTATGAATTCGAACAGCATACCCCCGATTCC38340.16897736082396075No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGA38100.16791959956684677No Hit
CATAAACAAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGAT37400.16483446256693096No Hit
CATGATGGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGATAAGGGTGGA36910.16267486666698985No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTC36180.1594575095099348No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC35430.1561520055814536No Hit
GTTAGATATGGGGAGTAGTGTGATTGAGGTGGAGTAGATTAGGCGTAGGT35410.1560638588100274No Hit
GATGCAGAGTGGGGTTTTGCAGTCCTTAGCTGTTACAGAAATTAAGTATT35090.15465351046720874No Hit
GCTTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACG34930.1539483362957994No Hit
CCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAGGGTGAGCATCAAAC34260.15099541945302286No Hit
GCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCTTGGAAAAAGG34140.15046653882446584No Hit
CCCCATTCCTCCCCACACTCATCGCCCTTACCACGCTACTCCTACCTATC33970.14971729126734343No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT33950.14962914449591727No Hit
ATTGGTGCGGGGGCTTTGTATGATTATGGGCGTTGATTAGTAGTAGTTAC33640.14826286953881168No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTC33630.1482187961530986No Hit
CTTGTCAGGGAGGTAGCGATGAGAGTAATAGATAGGGCTCAGGCGTTTGT32980.1453540260817482No Hit
GTTGATTAGGGTGCTTAGCTGTTAACTAAGTGTTTGTGGGTTTAAGTCCC32810.14460477852462578No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACG31720.13980077948189973No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAG31230.13764118358195865No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCG31170.13737674326768015No Hit
CTTCTATGATAGGGGAAGTAGCGTCTTGTAGACCTACTTGCGCTGCATGT30960.1364512021677054No Hit
CCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCTCAGGCTTCAACATC30780.1356578812248699No Hit
ACTTTAGGATGGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG30770.13561380783915683No Hit
CCCCTCACCCACTAGGATACCAACAAACCTACCCACCCTTAACAGTACAT30740.13548158768201757No Hit
GGTAAGGATGGGGGGAATTAGGGAAGTCAGGGTTAGGGTGGTTATAGTAG30540.13460011996775592No Hit
GCCTAACCCCTGTCTTTAGATTTACAGTCCAATGCTTCACTCAGCCATTT30020.13230830391067558No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGA29900.13177942328211859No Hit
AGGGAGAGCTGGGTTGTTTGGGTTGTGGCTCAGTGTCAGTTCGAGATAAT29820.1314268361964139No Hit
GCAATAATGAAGGGCAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGT29560.13028092816787376No Hit
GGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTTG29510.13006056123930834No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG29350.129355387067899No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA29010.12785689195365418No Hit
CCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGCTGACGCCATA28840.12710764439653177No Hit
GAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGG28830.1270635710108187No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTT28760.12675505731082712No Hit
CGTAGTAACAGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAG28740.12666691053940096No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCA28450.12538878235372153No Hit
CCTAGGGAGAGGAGGGTGGATGGAATTAAGGGTGTTAGTCATGTTAGCTT28380.12508026865372995No Hit
CCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATCCGTACTATA28340.12490397511087763No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGA28130.12397843401090287No Hit
CCATCATAGCCACCATCACCCTCCTTAACCTCTACTTCTACCTACGCCTA27370.12062885669670854No Hit
TTTGTTTATGGGGTGATGTGAGCCCGTCTAAACATTTTCAGTGTATTGCT27200.11987960913958613No Hit
AGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACACAA27110.1194829486681684No Hit
AGTAAGGGTGGGGAAGCGAGGTTGACCTGTTAGGGTGAGAAGAATTATTC27100.11943887528245531No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACT27070.11930665512531605No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTAC26980.11890999465389832No Hit
CCTATTTATGGGGGTTTAGTATTGATTGTTAGCGGTGTGGTCGGGTGTGT26920.11864555433961982No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC26560.11705891245394882No Hit
ACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAATTC26450.11657410521110491No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTAT26270.1157807842682694No Hit
AGCTATTAGAAGGATTATGGATGCGGTTGCTTGCGTGAGGAAATACTTGA25920.1142382157683115No Hit
TCTATTGATGAGGGTCTTACTCTTTTAGTATAAATAGTACCGTTAACTTC25560.11265157388264051No Hit
CCCGCTAACCGGCTTTTTGCCCAAATGGGCCATTATCGAAGAATTCACAA25500.112387133568362No Hit
GCTATGATGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGAT25490.11234306018264892No Hit
CCTAGATAGGGGATTGTGCGGTGTGTGATGCTAGGGTAGAATCCGAGTAT25470.11225491341122276No Hit
AGGAATGGGGTGGGTTTTGTATGTTCAAACTGTCATTTTATTTTTACGTT25090.1105801247541256No Hit
GGTGAGGGGTGGCTTTGGAGTTGCAGTTGATGTGTGATAGTTGAGGGTTG24930.10987495058271626No Hit
GGTGATTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAGAATAGGAGGT24890.10969865703986395No Hit
TCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCACC24830.10943421672558544No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT24800.10930199656844619No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGG24620.10850867562561069No Hit
AGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTC24590.10837645546847145No Hit
GGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAG24480.10789164822562754No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC24440.10771535468277518No Hit
GCTTACGACCCCTTATTTACCGAGAAAGCTCACAAGAACTGCTAACTCAT24390.10749498775420978No Hit
AGCATTAGCAGGAATACCTTTCCTCACAGGTTTCTACTCCAAAGACCACA24390.10749498775420978No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTTTGACTATTTCTGTATGTCTC24290.10705425389707895No Hit
GCCATCATGACCCTTGGCCATAATATGATTTATCTCCACACTAGCAGAGA24270.10696610712565277No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCAT24230.10678981358280046No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCT24170.10652537326852195No Hit
ATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCT24130.10634907972566962No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA24130.10634907972566962No Hit
GGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAG23870.10520317169712945No Hit
CAACACCCACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCT23790.10485058461142478No Hit
GCCAATGGTGAGGGAGGTTGAAGTGAGAGGTATGGTTTTGAGTAGTCCTC23720.10454207091143322No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG23470.10344023626860613No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGC23390.10308764918290146No Hit
CCTCGGAGCTGGTAAAAAGAGGCCTAACCCCTGTCTTTAGATTTACAGTC23320.10277913548290987No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGC23300.10269098871148372No Hit
CTGAAGCTTCACCGGCGCAGTCATTCCCATAATCGCCCACGGGCTTACAT23190.10220618146863981No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC23140.1019858145400744No Hit
GATGTGGGGAGGGGTGTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGT23100.10180952099722206No Hit
CCTCAGATAGGGGTCCCTTGATCACCATCCTCCGTGAAATCAATATCCCG23060.10163322745436973No Hit
CTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGCAG22990.10132471375437814No Hit
GGTCGAAGGGGGTTCGGTTGGTCTCTGCTAGTGTGGAGATAAATCATATT22760.10031102588297723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)