FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_145_S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_145_S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299227
Sequences flagged as poor quality0
Sequence length110
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA84807725.70532430778482No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC76542123.200010184203755No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC71815921.76749280967936No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC67299920.39868732888037No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT85080.25787858792377727No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT62830.19043854818113456No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCAC33250.10078118298619647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)