FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_145_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_145_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4162161
Sequences flagged as poor quality0
Sequence length110
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA107390425.801596814731575No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC95316422.900699900844778No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC89644421.5379462735824No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC86955720.89195972957317No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT104150.2502305893500996No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT78510.18862797474677218No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCAC42700.10259093773643066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)