FastQCFastQC Report
Wed 29 Mar 2023
HCMLCAFX5_n01_145_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCMLCAFX5_n01_145_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3814467
Sequences flagged as poor quality0
Sequence length110
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA98240425.75468604132635No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC85611622.44392204730045No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC82742521.691759294286726No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCAC80730821.164372374960905No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT92430.24231432595956393No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACT74500.19530906939291912No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCAC38790.10169179599666218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)