FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l02n02_lacz_20hpf_16_june.3520000003c691.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l02n02_lacz_20hpf_16_june.3520000003c691.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30052205
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC73250.010.3519745
TCTGACG93400.09.05819545
TATACCG54400.08.5640345
GTATAGA83400.08.4190891
TAGACCG52200.08.1057715
GTATAGG74300.07.96605971
TATACAC519000.07.91651745
GTATTAG91200.07.89644861
GTATAAG121800.07.7048711
TATACTG99400.07.53988745
GTATATA243350.07.4908361
CTTATAC673850.07.479723545
CCAAAGG72750.07.45529843
AGACCGT63200.07.4045116
ATCTGAC115800.07.325460444
CCTTTGG99550.07.256721
GTCTAGA34700.07.1989661
GTATACG51050.07.1416061
TACACTG92800.07.0817715
CCTACAC66750.07.0802563
Produced by FastQC (version 0.11.2)