FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l02n02_lacz_18hpf_19_may.3520000003c61a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l02n02_lacz_18hpf_19_may.3520000003c61a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5053016
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG10600.013.16146643
AGATCTC11450.012.38032438
TCTCGGT11800.011.82289441
GTAGATC12500.011.33992936
GACGAGT12550.011.29430129
CTGCCGA13350.010.78570124
CTCGGTG13400.010.74715842
CCGACGA14450.010.58774827
CCAAAGG10300.010.0502713
ACGCTGC16200.09.99944221
GAGTAGG6600.09.8879641
GGTGGTC14800.09.73043845
GTATAGG11400.09.6726191
TATAGGG12500.09.5415452
CTATAGG10850.09.5407151
ACTCCGC3104.6320893E-69.4348636
CGCTGCC16800.09.37447622
GCCGACG15950.09.30982526
GCTGCCG17750.09.252912523
TACGGGC4702.3137545E-99.09532628
Produced by FastQC (version 0.11.2)