FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l02n02_dn_20hpf_11_june.3520000003c65e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l02n02_dn_20hpf_11_june.3520000003c65e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18283486
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG34300.011.3517895
AGACCGT39700.09.5772816
TCTGACG67500.08.9670945
CTGACGC55850.08.82315645
GTATAAG86500.08.7158581
TATACAC387200.08.52506645
GTCTAGT24000.08.251881
GTATAGG49500.08.2291471
GTATATA154350.08.0922221
GTGTAGG39500.08.090451
TACACAT230950.07.979377345
TAGACTG29200.07.9390175
TATACTG63300.07.92891035
TATACCG36800.07.8895875
TAGGGAG19650.07.7885885
ATACACA321750.07.762604745
GTATAGA51550.07.68361231
CTTATAC533850.07.67948645
TCTATAC52700.07.6441193
TTATACA519700.07.542207745
Produced by FastQC (version 0.11.2)