FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l02n02_dn_18hpf_21_may.3520000003c5ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l02n02_dn_18hpf_21_may.3520000003c5ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27794776
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG42150.010.0356943
TCTCGGT44550.09.89896841
CTCGGTG44350.09.58854742
TAGACCG52050.09.0810585
AGACCGT57950.08.6189536
AGATCTC55000.08.50896438
GTATAAG153850.08.49918651
ACTAGTC41900.08.3766938
CTAGTCA41000.08.1763259
GTAGATC54700.08.10314136
GTATATA299250.07.96453761
GACGAGT56250.07.839615329
GAGTAGG33800.07.79054641
CGGTGGT62750.07.745144
CCGACGA58050.07.596485627
GTATAGA101500.07.5611371
GTATAGG88900.07.5442021
CTGCCGA57000.07.538954724
TATACCG64850.07.5316065
GTACTAC56000.07.47534943
Produced by FastQC (version 0.11.2)