FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l02n01_lacz_20hpf_16_june.3510000003c694.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l02n01_lacz_20hpf_16_june.3510000003c694.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30052205
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCGTGATATCTCGTATGCCGTC325370.10826826184634371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG77850.020.05734843
TCCGAGC81600.019.49378413
GAGACGT83150.019.130424
CGAGCCC83900.019.12028715
GTATGCC83250.018.94535842
CCGAGCC85400.018.86349114
CGAGACG85150.018.6546423
TGCCGTC85500.018.4996145
AGACGTC85950.018.48100925
GCCCACG86150.018.43810518
CGTATGC86250.018.36464741
TATCTCG85500.018.0521636
AGCCCAC91600.017.63582817
GAGCCCA94100.017.28683916
CTCCGAG93400.017.27186212
CCCACGA92600.017.25119
ATGCCGT91150.017.22947744
CTCGTAT92150.017.09125339
CCACGAG93450.016.99778420
TCGTATG94250.016.90133340
Produced by FastQC (version 0.11.2)