FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l02n01_lacz_18hpf_19_may.3510000003c61d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l02n01_lacz_18hpf_19_may.3510000003c61d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5053016
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11700.019.9996943
GTATGCC12800.018.28060342
ATGCCGT14450.016.34922644
CGTATGC14700.016.0708641
TGCCGTC15300.015.293879545
GCTACGC17150.013.51264129
CTACGCT17350.013.35687630
TCGTATG17700.012.96564340
CGCTATC17650.012.61994933
TACGCTA18050.012.58958231
TCTCGTA17900.012.56938738
CGCTACG18600.012.45923628
GACGCTA18600.012.45923626
TATCTCG18000.012.24956636
ACGCTAT18400.012.22782832
CTCGTAT18750.012.11957239
CGAGCCC19050.011.92871115
ACGCTAC19350.011.86004527
AGACCGG7450.011.4761039
CCGAGCC20200.011.47236514
Produced by FastQC (version 0.11.2)