FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l02n01_dn_20hpf_11_june.3510000003c651.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l02n01_dn_20hpf_11_june.3510000003c651.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18283486
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG45150.010.56450245
TAGACCG34450.010.38530355
TCTCCGA66550.08.99299845
AGACCGT40200.08.9558176
GTATATA151800.08.5720741
TATACAC395100.08.42232645
TATACCG36250.08.3798655
CCAAAGG43450.08.28681853
TAGGCCG13900.08.2559425
GTATAAG80850.08.130791
GTGTAGG42200.07.9488391
TACACAT233750.07.94096445
TAGACAG31850.07.9126125
GTCTATG53800.07.9087781
ACCGCTC23200.07.85530768
GTATAGG46750.07.84938961
GTATACG35550.07.78924561
ATACACA320000.07.762292445
CTTATAC538750.07.68007345
ATCTCCG79700.07.678590344
Produced by FastQC (version 0.11.2)