FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l02n01_dn_18hpf_21_may.3510000003c5f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l02n01_dn_18hpf_21_may.3510000003c5f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27794776
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC52550.08.52013619
CCGAGCC51800.08.42632320
GTATAAG150300.08.2982811
CTCCGAG51100.08.27757518
CGAGCCC52150.08.24034121
GTAGAGG55550.08.18155435
ACCGCTC30200.07.97155438
CGAGACG54500.07.9675929
GAGACGT54850.07.957767530
CCTAGAC15000.07.95147373
TAGACCG51550.07.90055275
GTATAGG86150.07.8920341
AGACGTA57800.07.707321631
GCCCACG60850.07.69075424
AGCCCAC58850.07.68450223
GTATATA308350.07.6443411
CGTAGAG57600.07.61694134
TATAGGG63050.07.6033952
CCTACAC74400.07.59213353
GTATAGA98850.07.53856471
Produced by FastQC (version 0.11.2)