FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l01n02_lacz_18hpf_14_may.3520000003c641.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l01n02_lacz_18hpf_14_may.3520000003c641.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42509647
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC140600.012.80350145
TCTGACG186800.011.15470845
TAGACCG84200.011.0625585
AGACCGT98150.09.5591046
TATACAC1123400.08.94357445
ATCTGAC234050.08.90284844
ATACACA878900.08.4616745
TATACCG90450.08.4574165
CCAAAGG89400.08.3802153
GTATATA415800.08.2284981
GTATAAG201350.08.1554351
TATACTG164000.08.0393525
GTATAGG111950.08.0172371
CTATACT130900.07.90652234
TTATACA1482500.07.856401445
CTTATAC1537350.07.820535745
CATAGAC132650.07.7849153
ACCGTGC50750.07.75883348
TACACAT633400.07.70203245
GTATAGA133900.07.61015561
Produced by FastQC (version 0.11.2)