FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l01n02_lacz_15hpf_23_apr.3520000003c60d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l01n02_lacz_15hpf_23_apr.3520000003c60d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13468615
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG20750.010.73654843
CTCGGTG20750.010.08547442
TAGACCG29900.09.9323855
GTATAAG87600.09.7579671
TCTCGGT21900.09.35040341
AGACCGT32400.09.3050146
AGATCTC25200.09.0188438
TATACTG65950.08.6991385
TCCCGAG14400.08.59379321
CCGGGCT13650.08.57099314
GTAGATC25900.08.51437836
GTATATA163250.08.3502451
TATACCG34800.08.2752585
CTGCCGA26650.08.27411624
CCGACGA26900.08.196609527
TATACAG81250.08.1963255
TCCTATA35200.08.1808262
GCTGCCG27850.08.16015823
TACACTG53450.08.0817295
AACCGCG14800.08.0576227
Produced by FastQC (version 0.11.2)