FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l01n02_dn_20hpf_9_june.3520000003c684.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l01n02_dn_20hpf_9_june.3520000003c684.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9940603
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGTA11500.011.34647111
GTATAAG45250.09.9921751
CCGCTCG16000.09.9828829
ACCGCTC16650.09.8649278
GCGGTAG9300.09.192451511
AGACCGG17900.09.1746049
AGTACCG8700.09.0514175
TAGACCG16000.08.8590755
ACTGACC20150.08.7097338
TATACCG19450.08.5601225
ACTAGTC12650.08.5376048
GTATATA100300.08.5224661
TATAGGG29100.08.5045672
CCGGCAG19500.08.42236812
GGAGACC18750.08.400187
GTCTTAG11000.08.3844341
CGGCAGA20200.08.35325713
TATACTG31550.08.3436165
TCCTAGC5151.19343895E-88.3003972
AGACCGT18250.08.26003556
Produced by FastQC (version 0.11.2)