FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l01n02_dn_15hpf_14_apr.3520000003c627.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l01n02_dn_15hpf_14_apr.3520000003c627.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10279441
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC362910.3530444894814806Illumina Single End PCR Primer 1 (95% over 22bp)
GTATAAGAGACAGCTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTA123280.11992870040306666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG51700.033.211443
CGGTGGT56450.030.33590744
GGTGGTC58000.029.21496645
CTCGGTG66100.025.87259542
TCTCGGT68150.025.02829241
AGATCTC74400.023.16785238
ATCTCGG75150.022.87641740
GTAGATC74900.022.86253436
GATCTCG80250.021.70298839
GTGTAGA84800.020.1917734
AGTGTAG90900.019.03478833
CTGCCGA92200.018.5469524
CCGACGA92950.018.25072127
GCTGCCG94800.018.15730523
GCCGACG93950.018.12909926
CGAGTGT95500.018.02324931
GACGAGT94050.017.98950629
TGTAGAT97050.017.92121335
CGCTGCC95400.017.90150522
TAGATCT97750.017.88686637
Produced by FastQC (version 0.11.2)