FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l01n01_lacz_18hpf_14_may.3510000003c644.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l01n01_lacz_18hpf_14_may.3510000003c644.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42509647
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG81350.014.65892143
TGCCGTC87000.013.93974345
GTATGCC92950.012.85340242
ATGCCGT96400.012.37040944
CTCCGAG146750.012.06651445
CCGAGCC106250.011.81566914
CGAGCCC102500.011.80895415
CGTATGC103150.011.73505141
GCCCACG105700.011.53658818
GAGAGGA107750.011.17094931
AGCCCAC109300.011.11543917
GAGCCCA110600.011.00513116
CAGAGAG109500.010.97188529
CCACGAG110900.010.95507320
CGAGACC107250.010.92935423
CCCACGA112200.010.80808919
CCAGAGA113400.010.63422428
AGAGAGG111850.010.6206630
TCCGAGC113150.010.59804313
TCGTATG116000.010.57042840
Produced by FastQC (version 0.11.2)