FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l01n01_lacz_15hpf_23_apr.3510000003c600.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l01n01_lacz_15hpf_23_apr.3510000003c600.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13468615
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC18900.012.85737145
TATGCCG20350.012.05176643
ATGCCGT21650.011.12028944
GTATGCC25500.09.97039542
TAGACCG27550.09.7211755
TAGACGG9200.09.540485
CGTATGC28300.09.222341
GTATAAG83400.09.153051
GTATACG30550.08.9925071
GTATAGG46200.08.9682571
TACACTG54150.08.6448665
TATACTG64250.08.6169865
AGACCGT31700.08.5903636
CCTACAC36450.08.5206943
TCGTATG31450.08.51296940
CCGAGCC31650.08.38805914
GTAGAGG30350.08.37679229
CGTAGAG29550.08.37516128
TATACCG32100.08.3432525
TACACCG25700.08.3192445
Produced by FastQC (version 0.11.2)