FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l01n01_dn_20hpf_9_june.3510000003c687.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l01n01_dn_20hpf_9_june.3510000003c687.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9940603
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14550.016.0826243
TCCGAGC16050.015.27905213
GAGACTA15850.014.90414824
ACTAAGG16300.014.35465927
CTCCGAG16950.013.93684812
CGTATGC17650.013.76748541
CCGAGCC17950.013.66177114
GTATGCC17850.013.48731442
GACTAAG18850.013.12890326
CTAAGGC18400.012.960928
ATGCCGT18750.012.96011744
CGAGCCC18350.012.7509415
CGAGACT18250.012.69759723
GCGAATC17500.012.59897333
AGCCCAC18950.012.34721717
GAGCCCA20050.012.11865116
GCCCACG20150.011.83520418
GGCGAAT19350.011.51068932
TGCCGTC20150.011.50134545
CACGAGA22200.010.94499921
Produced by FastQC (version 0.11.2)