FastQCFastQC Report
Mon 16 Nov 2015
HCM7MBCXX_l01n01_dn_15hpf_14_apr.3510000003c62a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCM7MBCXX_l01n01_dn_15hpf_14_apr.3510000003c62a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10279441
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT334100.3250176736264161No Hit
CTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGG129830.12630064222363843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT65350.024.65212845
TCTCGTA63650.024.46215244
CTAGGCA79000.021.41627534
ACTAGGC79700.021.05880233
TGATCTC74250.020.8782941
ATCTCGT75200.020.7945643
TAGGCAT85500.019.89339335
GATCTCG78900.019.79040342
CATGATC80000.019.51743139
ATGATCT80850.019.28441240
GGCATGA89050.019.10033837
GCATGAT90050.018.2136538
AGGCATG93450.018.10471536
CTCCGAG98350.017.4314618
TCCGAGC99500.017.25260419
CGAGACT98000.017.14935129
CCGAGCC100150.017.11816420
GAGACTA98450.017.1166730
GCCCACG99550.017.08573724
GACTAGG99300.017.03812432
Produced by FastQC (version 0.11.2)