FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_nw4-mss.3520000003ef42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_nw4-mss.3520000003ef42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117997
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA108050.068.6422245
GGGGGGG449600.055.449253240-244
GGGGGGA120750.052.50649245
GGGGGAG123900.044.09124245
GGGGGCC64700.038.211178245
GGGGGAC49650.036.943806245
AGGGGGG122550.035.206024240-244
CGGGGGG68300.030.358873240-244
GGGGGGC72850.030.104828245
CCGGGGG47500.030.05332240-244
CGGGGAA28100.026.961887245
GGGGGCG47050.025.425215245
AAGGGGG101550.023.952616240-244
GGGGGCA72000.022.706718245
GAGGGGG127050.021.59319240-244
GCGGGGG49950.020.436546240-244
ACTGATC63150.020.4037068
TGGGGGG103000.019.821465240-244
GGGGGGT56950.019.745058240-244
GGGGGTT47050.019.492664245
Produced by FastQC (version 0.11.2)