FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_nw3-mss.3520000003ef1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_nw3-mss.3520000003ef1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10033
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGGAC100.0023460384208.8353
GACAAGG302.482926E-6139.223347
ACTACGC100.007929334139.22334205-209
AGACAAG358.1446953E-489.500716
GGGGGAG400.00697139552.20875240-244
GGGGGGG1501.2732926E-1146.407776230-234
CCCCCCC658.682557E-442.837948235-239
AAAAAAA6850.020.324575245
Produced by FastQC (version 0.11.2)