FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_lhmp-extraction-control-mss.3520000003f0b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_lhmp-extraction-control-mss.3520000003f0b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5900019
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG504850.054.591248240-244
GGGGGAA121200.053.68494245
GGGGGGA127950.042.42932245
GGGGGCC75750.036.887814245
GGGGGAG135550.035.927563245
AGGGGGG134850.033.924877240-244
GGGGGGC87250.033.398342245
CGGGGGG75900.032.398396240-244
GGGGGCG54450.030.05754245
CGGGGAA30900.028.420498245
GGGGGTA38000.027.312233245
GGGGGTT60150.027.209196245
CGCGGAA5950.00887163226.835552245
GGGGGAC61550.025.94176245
CGGCGCC15558.991265E-925.670664245
CCGGGGG57550.024.832605240-244
CGGGGGC37500.024.482967245
GGGGGGT65550.023.74977245
AAGGGGG111750.022.933592240-244
CCCCCCC403350.022.76215245
Produced by FastQC (version 0.11.2)