FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_53115004-1-mss.3520000003f0ab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_53115004-1-mss.3520000003f0ab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5136869
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG331950.055.219856240-244
GGGGGGA94200.053.107245
GGGGGAA87550.048.455418245
GGGGGAG106850.034.833817245
CGGGGGG60250.034.276394240-244
GCGCGAA6002.2712903E-433.351192245
AGGGGGG101600.031.750006240-244
CCGGGGG46850.028.190763240-244
GGGGGCC60150.026.614418245
GGGGGGC71600.024.482939240-244
GGGGGAC47750.024.306211245
GCGGGCC16952.1862434E-823.611467245
CGGGGAA25900.023.178436245
GCCCGCG9200.00275700121.750778245
CCCCCCC319450.021.448818240-244
GGGGGGT48700.021.2028240-244
GGGGGCA66550.021.048086245
CGGGGCC30600.020.926239245
GCGGGGG46900.020.651186240-244
CGGGGCA25752.1464075E-1020.204994245
Produced by FastQC (version 0.11.2)