FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_52370180-2-rep-mss.3520000003f081.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_52370180-2-rep-mss.3520000003f081.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4893062
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA82350.060.854412245
GGGGGGA89800.056.691612245
GGGGGGG323350.054.243042240-244
GGGGGAG94350.038.360535245
AGGGGGG95800.037.447197240-244
CGGGGGG52500.033.63582240-244
GCGCGAA5100.004252706731.19428245
GGGGGCC52100.030.53567245
GGGGGCG34350.030.104525245
CGGGGCC24800.028.867289245
CCCCCCC267500.026.316893245
CCGGGGG39450.026.212687245
GGGGGGC56650.025.97688245
GGCGGAA16958.276402E-1025.811197245
GCGGGGG40000.022.869307245
GAGGGGG98100.022.055058240-244
AAGGGGG76250.021.385645240-244
CCCGGGG40400.020.673605240-244
GGGGGTT40850.020.44619245
CGGGGAA23803.9835868E-1020.053467245
Produced by FastQC (version 0.11.2)