FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_52370180-2-mss.3520000003efd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_52370180-2-mss.3520000003efd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5162138
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA98850.071.46358245
GGGGGGG440400.056.342613240-244
GGGGGGA105350.054.10066245
GGGGGAG112350.051.444405245
GGGGGCG37200.037.763638245
AGGGGGG116100.036.6462240-244
CGGGGGG59300.032.083294240-244
GGGGGTA31500.030.580843245
CCGCGAA5300.00490914430.292341245
CCCGGCG13451.8026185E-929.8419245
GGGGGCC57600.027.873163245
GGGGGGC65250.027.680937245
CCGGGGG40900.026.889488240-244
AAGGGGG93850.024.634485240-244
GAGGGGG116800.024.60512240-244
GGGGGCA61800.024.030457245
CGCGGGG13800.023.26803245
CCCCCCC320700.023.153757245
GGGGGGT54400.022.87239245
GGGGGAC44350.022.625341245
Produced by FastQC (version 0.11.2)