FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_52260024-1-mss.3520000003f067.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_52260024-1-mss.3520000003f067.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4734761
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA81750.057.57151245
GGGGGGG280000.053.36133240-244
CGGCGAA5958.7198714E-846.92393245
GGGGGGA83650.046.727596245
GGGGGCG30050.034.50978245
CGCGGCC10505.673428E-934.187435245
AGGGGGG86800.033.912006240-244
GGGGGGC50200.030.98662245
GGGGGCC42400.030.102144245
CGGGGGG45200.029.473038240-244
GGGGGAG83900.028.523485245
CGGGGAA21600.027.698156245
GGGGGTA26350.027.246155245
GGGGGAC41800.026.717455245
CGGGGCG9551.1888795E-425.058855245
GGGGGGT40400.024.681522245
CCCCCCC231300.022.244743245
CCGGGGG32350.022.192772245
AAGGGGG77750.022.161554240-244
CGGGGCC18054.413414E-822.097143245
Produced by FastQC (version 0.11.2)