FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_52150223-1-mss.3520000003f031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_52150223-1-mss.3520000003f031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3447976
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG173300.052.829624240-244
GGGGGGA59950.043.196182245
GGGGGAA58350.038.91842245
AGGGGGG63100.035.988743245
GGGGGCC32950.035.06419245
GGGGGCG23900.035.00601245
CGGGGGG32050.031.076925240-244
CGCGGAC5200.00463719830.646288245
CGCGGGA6654.262887E-429.955013245
GGGGGAG62650.028.616245245
GGGGGAC31050.028.228142245
AACGGGG17153.092282E-1127.876503245
GGGGGGC36700.027.139082245
CGGGGAA16907.8216544E-1025.931473245
GCGGGAA13908.7702574E-825.795794245
GAGGGGG66300.023.796207240-244
CCGGGCC17202.6771886E-823.16289245
CCCCGCG8750.002114831222.765812245
GGGGGCA42300.022.604353245
GGGCGAA12703.2605738E-521.959148245
Produced by FastQC (version 0.11.2)