FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_50331580-1-mss.3520000003f017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_50331580-1-mss.3520000003f017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5095611
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA95650.051.69754245
GGGGGGG338750.051.06814240-244
GGGGGGA100100.048.204155245
GGGGGCG41500.040.358444245
GGGGGAG101650.036.484528245
CCGCGAA5651.629556E-435.29025245
CGGGGGG59200.032.738163240-244
GGGGGCC57500.031.902386245
AGGGGGG101100.031.871313240-244
GGGGGGC67750.026.134455240-244
CGGGGCG12452.2456334E-725.624405245
GGGGGAC50300.025.369688245
CCGGGGG42600.023.402927240-244
CGGGGCC25609.094947E-1221.80827245
GGGGGCA70300.021.555666245
GGGCGAA16905.7341094E-721.236795245
CCCCCCC303650.020.75026240-244
GGGGGTT46150.020.73828245
GGGGGGT50650.020.470436245
AAGGGGG81100.020.357258240-244
Produced by FastQC (version 0.11.2)