FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_50220371-2-mss.3520000003efff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_50220371-2-mss.3520000003efff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5219414
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG387200.053.43383240-244
GGGGGAA104250.052.748135245
GGGGGGA106400.047.18816245
GGGGGAG114450.036.5576245
GGGGGCG44450.035.858517245
AGGGGGG112500.031.879168240-244
CGGGGGG60400.031.667109245
GGGGGCC61800.030.949732245
GGGGGGC72450.030.250212245
CGGGGGA27400.024.723076245
GGGGGAC49950.023.932598245
CGGGGAA28450.023.810623245
GCGGGGG46500.022.452532240-244
CCCCCCC307200.022.440317245
CCGGGGG45600.021.32269240-244
CCCGGAA22559.476935E-1021.205027245
CCGGCAA15055.022848E-621.181545245
AAGGGGG94250.020.548111240-244
GGGCGGG46650.020.500465245
GGGGGGT53600.020.072575245
Produced by FastQC (version 0.11.2)