FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_44065-mss.3520000003efbb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_44065-mss.3520000003efbb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4544405
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA94500.067.06965245
GGGGGGG410850.056.237278240-244
GGGGGGA100750.050.24807245
GGGGGAG103300.043.991146245
GGGGGCC55600.042.299763245
AGGGGGG111700.035.61694240-244
CGGGGGG58000.032.85331240-244
GGGGGGT52250.029.753565245
GGGGGCG36750.029.286476245
CCGGGGG43400.027.188246240-244
GGGGGTT44300.026.094862245
GGGGGAC44200.025.25204245
CGGGGAC19001.10958354E-1025.176096245
CGGGGAA22451.8189894E-1224.858356245
GGGGGGC63900.023.705193245
CCCGGGG45600.023.428755240-244
CCCCCCC287650.022.58832245
CGGGGCC26700.022.394466245
GGGGGAT41150.022.28018245
AAGGGGG88100.022.171719240-244
Produced by FastQC (version 0.11.2)