FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_35008-2-mss.3520000003ef92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_35008-2-mss.3520000003ef92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822293
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA88650.064.588234245
GGGGGGG357700.057.49085240-244
GGGGGGA93500.056.527332245
GGGGGAG97600.044.71702245
GGGGGCG31750.040.35547245
AGGGGGG97350.038.57976240-244
CGGGGCG9256.865412E-834.629353245
GGGGGCC44500.033.291843245
GGGGGTT39850.032.152725245
CCGGGGG34100.031.703377245
CGGGGGG49650.027.902908240-244
GCGGGAC10356.4320902E-627.080324245
CGCCGCG6000.00910364426.693459245
GAGGGGG96900.025.53626240-244
CCCCCCC244750.025.030233245
GGGGGGC56500.024.803658245
GGGGGTA30800.024.700117245
AAGGGGG80150.024.278698240-244
AGGGGAA130450.023.634302245
GTCCTAG26850.022.2656611
Produced by FastQC (version 0.11.2)