FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_35008-1-mss.3520000003ef85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_35008-1-mss.3520000003ef85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1267152
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGG2000.00412663559.88663245
GGGGGGA21450.057.69963245
GGGGGAA22300.055.500317245
GGGGGGG77300.054.95419240-244
GCGGGCC2250.006577120653.23256245
TTGCGCG654.5593362E-449.137753205-209
CCGAAAA5854.1347503E-640.948128245
CGGGGAA4957.41703E-540.3277245
GGGGGAG21100.039.735207245
GGGGGGC12650.037.87297245
GGGGGCC10952.0918378E-1036.46066245
AGGGGGG22650.035.60589240-244
GGGCGGG8050.034.71689245
GGGGGCG6855.620677E-1029.141912245
GGGGGTT9804.2896772E-628.517445245
CGATCGA1150.0079568627.441145215-219
CGGGGGG11700.026.616282240-244
GGGGCCC9108.488438E-526.323797245
GGGGGGT10650.026.241405245
CCTATAC8700.026.0657143
Produced by FastQC (version 0.11.2)