FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_34310-1-mss.3520000003eef0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_34310-1-mss.3520000003eef0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43256130
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA835400.065.56186245
GGGGGGG4379700.057.6201240-244
GGGGGGA917650.057.291096245
GGGGGCC618350.045.999584245
GGGGGAG981600.042.000397245
GGGGGGC723250.037.394566245
AGGGGGG1018800.036.725925240-244
GGGGGCG541900.034.501263245
CGGGGGG746250.033.233406240-244
GGGGGAC433500.029.766083245
GGGGGGT499750.028.61795245
CCGGGGG567750.026.639797240-244
GGGGGTT425150.025.558477245
AAGGGGG792650.025.1191240-244
CCCCCCC3356350.024.638336245
GGGGGCA578050.023.98128245
GGGGAAA1063250.023.069662245
GAGGGGG1030900.022.76276240-244
TGGGGGG794100.022.598106240-244
CCGGGCC387350.022.277092245
Produced by FastQC (version 0.11.2)