FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_34302-1-mss.3520000003eee3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_34302-1-mss.3520000003eee3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578937
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG275650.054.270786240-244
GGGGGAA67550.054.071827245
GGGGGGA70900.044.23738245
GGGGGAG75650.037.786102245
GGGGGCG25200.036.235634245
AGGGGGG79050.035.45815240-244
CGGCGGG8701.776134E-631.943857245
GGGGGGC42600.031.686762245
GGGGGCC36650.031.414675245
GGGGGTT32900.030.168427245
CGGGGGG35700.028.914679240-244
GGGGGTA22900.026.005445245
CCGGGGG26900.025.680899240-244
GGGGGCA45750.025.166073245
CGGGGAC11401.45342765E-524.378206245
CGGGGGA18050.024.19491245
AAGGGGG67050.023.684803245
GGGGGGT39000.023.413794245
CGGGGAA17252.8650902E-823.01545245
GCGGGGG27850.022.808846245
Produced by FastQC (version 0.11.2)