FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_34298-1-mss.3520000003eec9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_34298-1-mss.3520000003eec9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3050824
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA50950.058.749893245
GGGGGGG168700.053.70327245
GGGGGGA50000.052.682205245
AGGGGGG54200.041.236206245
GGGGGCG19700.040.518536245
CGGGGAA14300.039.07347245
CGACGCC4450.00214302435.87484245
GGGGGAG54250.035.312744245
CGGGGGG26050.031.560106240-244
GCGGGAA11701.6174454E-830.70058245
CCGGGGG18650.027.391138240-244
GGGGGGC30750.027.256128245
CGGGCCC12006.905466E-726.607172245
CCCGGGG19500.026.607172245
GGGCGAA9309.880876E-525.748877245
AAGGGGG48300.024.12763240-244
GAGGGGG56350.024.081024245
CCCCCCC159200.024.06679245
GGGGGTA18602.3283064E-923.603136245
GGGGGAC25005.456968E-1222.350025245
Produced by FastQC (version 0.11.2)