FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_34274-2-mss.3520000003ee93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_34274-2-mss.3520000003ee93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4020752
Sequences flagged as poor quality0
Sequence length251
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA68400.059.638783245
GGGGGGA68300.057.383896245
GGGGGGG255200.057.075844240-244
GGGGGAG67950.044.73102245
GGGGGCC33150.044.63781245
CGGGGGG34200.039.759186245
GGGGGCG24200.038.009933245
AGGGGGG74700.036.620865240-244
GGGGGGT35900.028.964336245
GGGGGTT32900.026.743084245
CCGGGGG24300.026.662556240-244
CGGGGGA18000.026.66204245
CGGGGAA16051.2332748E-824.917797245
CCCCCCC206000.024.655918245
GGGGGGC39600.024.238688240-244
GGGGGCA40300.023.817207245
AAGGGGG61150.023.545023240-244
GGGGGAC31750.022.673233245
GGGGGAT28700.022.295786245
CGGGGAC11053.1364328E-421.715693245
Produced by FastQC (version 0.11.2)