FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n02_34271-1-mss.3520000003ee79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n02_34271-1-mss.3520000003ee79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41267722
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAA692850.068.88791245
GGGGGGA751300.059.385365245
GGGGGGG3005550.057.995552240-244
GGGGGCC414200.044.70529245
GGGGGAG773250.040.771645245
AGGGGGG810650.037.857235240-244
GGGGGGC491750.036.194412245
GGGGGCG346300.034.456387245
CGGGGGG472000.034.175137240-244
GGGGGAC334750.031.115082245
GGGGGTT342900.027.815153245
GGGGGGT381900.026.75108245
CCGGGGG354750.026.05386240-244
AAGGGGG653000.025.496881240-244
CCCCCCC2455350.024.005909245
GAGGGGG813350.023.865583240-244
TGGGGGG633300.022.937592240-244
GGGGGTA235850.022.673573245
GGGGGCA454400.021.60468245
GCGGGGG392000.021.073671240-244
Produced by FastQC (version 0.11.2)