FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_nw4-mss.3510000003ef45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_nw4-mss.3510000003ef45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5117997
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA81650.017.9438931
GTATAGA43450.016.578831
ACTGATC83850.016.158358
GTACGGG5303.4857972E-416.1255231
GTATAGG31650.015.4304261
TATACAG62500.015.0395365
CCTATAC34300.014.9481493
GTATTAG50750.014.9158251
CTGATCA91300.014.8398429
GTATATA85050.014.6425751
CTAGGAC46200.014.5329223
GTGTAGA54450.014.350721
TATACTG52950.014.2938595
GTCCTAC29200.014.2163371
GTATAGC25300.013.9948781
GTCCTAA41900.013.9868151
GTCTAAG44600.013.9613341
CCGTACC12607.659764E-913.564064
GTGTAAG59800.013.4751741
TATATAC91400.013.3562973
Produced by FastQC (version 0.11.2)