FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_lhmp-extraction-control-mss.3510000003f0ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_lhmp-extraction-control-mss.3510000003f0ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5900019
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG61300.017.923715
GACCGCG4851.7740874E-417.6176097
GTATAGA51750.017.2227521
GTATTAG60700.016.6947331
GTGTATA92350.016.6580311
GTATATA103450.015.6968041
CCGTCCG7001.0321566E-515.6937899
ACCGCGT5000.004399659614.6475368
GTCCTAG47500.014.1370611
ACTGATC85750.013.2382978
TATATAC110950.013.094133
TCCGTAC14002.3828761E-913.0803733
TACACTG91650.013.0538775
ATATACT80800.012.9939244
GTATAAG50800.012.9783691
GGATCGT18850.012.950968
TTATACT72850.012.7361244
ATACTGT89150.012.735256
GTGTAGG50850.012.7255041
TCTATAC54800.012.6984653
Produced by FastQC (version 0.11.2)