FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_53115004-1-mss.3510000003f0ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_53115004-1-mss.3510000003f0ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5136869
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA88800.018.286671
GTGTATA80550.017.4312381
GTATAGG30050.017.0647831
TATACTG50350.016.971765
GTGTAGA53700.016.3702071
ACTGATC83150.015.7073648
GTACGGA16550.015.4923491
GTATTAG48450.015.3720581
GTGCTAG47550.014.89271
CTGATCA94400.014.7405839
CCTATAC36150.014.5212963
GTATACA62050.014.3640541
GGATCGT16150.014.360288
GTGTAGG43700.014.2490091
GTGTAAG58700.014.1438231
CTATACT47100.013.9961834
GTATATG75150.013.6472851
GATCGTT17900.013.6382549
TCTATAC46350.013.4327733
GTATTAA51000.013.4064511
Produced by FastQC (version 0.11.2)