FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_52370180-2-rep-mss.3510000003f084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_52370180-2-rep-mss.3510000003f084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4893062
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG48400.019.172831
GTATAGG29200.018.398731
ACTGATC90150.018.008228
GTGTATA84950.017.8228171
GGATCGT21150.017.3139348
GTACGGA19100.017.2603021
TACACTG77850.016.1523065
TATACAG60250.016.007595
GTATATA93950.015.8555411
GTATAGA43300.015.791311
GCTATAC25600.015.7379293
CTGATCA96650.015.6604679
GATCGTT23700.015.4510429
GCACTGA106450.015.2532316
AGGATCG26200.014.9086387
GCTAGAC57050.014.552143
ACGGACC18151.4551915E-1114.502476245
GTGTAGA51200.014.3086711
GTGTAAG55550.014.2872071
GTATAAG42800.014.2640951
Produced by FastQC (version 0.11.2)