FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_52370180-2-mss.3510000003efd8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_52370180-2-mss.3510000003efd8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5162138
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA91100.017.824951
TCCGTAC16350.016.4268263
GTATTAG44600.016.4252281
GTATAAG45350.015.8843611
GTGTATA84750.015.8470341
GTATAGG31700.015.7913621
ACTGATC81100.015.6527148
CCGTACC14050.015.6402934
GTCTTAG42200.015.6234281
GTATTAA50250.015.0643581
GTATAGA46400.014.7355061
TATACTG51400.014.7256035
GTGCTAG44400.014.0243331
CCGTATA7051.9225791E-413.8509039
GTGTAGG39850.013.7872991
CTGATCA86900.013.7652329
CTGTCGC20500.013.6946589
TCTATAC45150.013.2491213
TTCCGTA26450.012.9235282
GACCGCT9551.34922575E-512.7812647
Produced by FastQC (version 0.11.2)