FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_52260024-1-mss.3510000003f06a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_52260024-1-mss.3510000003f06a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4734761
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACC4454.0904584E-420.880527245
GTATATA85700.018.2344021
GTATAGA43900.017.2420751
GTATTAT58400.016.3058781
GTATTAG47900.016.0571121
TATATAC89350.015.4386593
TATACTG51700.015.347565
TCGTATT15703.7471182E-1014.795917245
TTATACT63050.014.7146394
GGATCGT16800.014.5314758
GTATAGG29750.013.9525741
GTCTAAG39150.013.72092151
GTGTATA76550.013.5561431
CTATACT49050.013.4392764
ATATACT69350.013.3779094
GATCGTT18250.013.3769219
TATACTA52400.013.2788395
CTGTCGC21250.013.2116769
TATAATA78250.013.1045052
GTATAAT50550.013.0417241
Produced by FastQC (version 0.11.2)