FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_52150223-1-mss.3510000003f034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_52150223-1-mss.3510000003f034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3447976
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA62900.019.0233481
GTATTAG36050.018.966811
GTATAGG20800.018.1974241
TATACAG46650.017.2716795
TATACTG38300.016.5747435
CTAATCG5253.2479063E-416.2755959
TCTATAC33150.016.203833
GTGTAAG37400.015.9969061
GCCGTAG4850.003585599815.1050111
GTATAAG29850.014.7254881
CCCTACG6651.1685395E-414.6864512
ATACTGT56700.014.6409116
TATTAGG36750.014.6165162
GTGTATA58850.014.3157521
GTATAGA31650.014.2737991
TAAGGCG6059.525235E-414.1248855
CTATACT32300.013.9843234
CCGTACC8755.461612E-613.9519274
TAATACT42950.013.9275644
GTGCTAG32600.013.8578441
Produced by FastQC (version 0.11.2)