FastQCFastQC Report
Mon 30 Nov 2015
HCJFNBCXX_l02n01_50331580-1-mss.3510000003f01a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCJFNBCXX_l02n01_50331580-1-mss.3510000003f01a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5095611
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG54100.017.1498745
CCTATAC33300.016.1311683
GTATTGG44800.015.5344861
GTATAGA43600.015.4019691
CTATACT48350.015.1496874
TCTATAC45900.014.8947343
GTGTATA82900.014.87533951
GTATATA96250.014.4611941
GTATTAT63850.014.150471
GTATAAG48600.014.068631
GTCCTAA42800.013.9782361
ACTGATC72700.013.7680668
GTGTAAG57900.013.706761
TAGACTG65100.013.5019385
GTATTAA57700.013.1194581
TCGTATT14303.9410406E-713.002096245
GTATTAG51000.012.9277711
GTGTAGG43450.012.9261171
GTATAGG30350.012.87334251
ATATACT73100.012.8594454
Produced by FastQC (version 0.11.2)